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Insert the needle and draw out until 3ml, then hold on to the plunger and remove the needle

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The pathogenic allele of GRM7 changes the synaptic autoregulation of glutamate in the synaptic fissure of the sensory cells of the IHC and auditory neurons, leading to an increase in the level of glutamate in the fissure, followed by the death of excitotoxic neurons and/or sensory cells [49]