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Anti-Mu and anti-Sigma probed membranes were incubated in a secondary antibody diluted at 1: 30 000 in TTBS (anti-Rabbit IgG, conjugated with alkaline phosphatase, produced in Goat, Sigma-Aldrich) and immunogenic proteins visualized using BCIP/NBT (5-bromo-4-chloro-3-indoyl phosphate/nitro blue tetrazolium) liquid substrate system (Sigma-Aldrich)

Light protection, aseptic technique on every draw, fresh needles per puncture, and upright storage are the handling practices documented to maintain product quality through the full window

PinheiroRTSouzaLDMTrettimJPde MatosMBPinheiroKATda CunhaGKet alAntenatal depression: efficacy of a pre-post therapy study and repercussions in motor development of children during the first 18 months postpartum

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However, we are also aware of the potential risks and complications associated with IV therapy, such as bruising or infection at the insertion site, or more severe issues like a blocked or damaged vein