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Get the timing right and the therapy does its job

Experiments with [5- 13 C] glutamine Cells were incubated in 2-well Lab-Tek chambers for 2022 h in either standard cell culture media supplemented with 10% FBS and penicillin (50 U/mL)-streptomycin (10 g/mL) or glutamine-free RPMI-1640 media (Corning, VA) supplemented with 1.5 mM [5- 13 C] glutamine, 10% FBS, and penicillin (50 U/mL)-streptomycin (10 g/mL) at 37C/5% CO 2

A final set of particles (numbers given in Table 1) were selected from the best-aligned 3D class averages for high-resolution 3D auto-refinement with the application of icosahedral symmetry throughout