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Sachs J, Dhl K, Weber A et al (2019) Novel 3,4-dihydroisocoumarins inhibit human P-gp and BCRP in multidrug resistant tumors and demonstrate substrate inhibition of yeast Pdr5

In most cases, the intein self-cleavage reaction is essentially complete in 4 hours, keeping the time to complete the entire procedure on par with conventional chromatographic protein purification processes

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Suv ML, Rheinbay E, Gillespie SM, Patel AP, Wakimoto H, Rabkin SD, et al

the present inventors recognize a solution to prevent in vivo target protein cleavage using split-inteins whose catalytic residues are split between two peptide chains: the N-terminal intein (IN) and the C-terminal intein (IC)