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Finally, GIRK channels expressed in HEK293 cells demonstrate basal K + currents that can be reduced by the application of a voltage-activated phosphatase (Dr-VSP), implying that there is a PIP 2 -mediated basal current in cells ( Mutagenesis studies on residues interacting with PIP 2 provide molecular insights into PIP 2 -dependent activation of GIRK channels

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Once reconstituted, store at 2-8C and use within 28 days