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For an accurate assessment of GSH concentration, the absorbance was measured against a sample in which, instead of 108 l of the supernatant from reaction mixture I, 108 l of the mixture are added, collected previously in the same way, but without GSH (90 l of Tris-HCl buffer with NaN 3 and EDTA + 10 l of phosphate buffer + 4 l H 2 O 2 solution + 20 l trichloroacetic acid solution)

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10.1073/pnas.150084897 56 NorrgrdM

Electrochemical reactions of highly active nitroxyl radicals with thiol compounds