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The freeze-thaw damage to the peptide structure cannot be reversed, and the solution may look perfectly fine while being significantly degraded

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Some investigators found a compensatory increase of other vitamin B 6 derivatives in maternal circulation corresponding to the fall of PLP (Reference Barnard, de Kock and Vermaak61) and unchanged urinary excretion of the catabolic product 4-pyridoxic acid (Reference Trumbo and Wang60) leading to the conclusion that there are no substantial changes in vitamin B 6 status in pregnancy, however, this remains uncertain as others have failed to confirm these findings (Reference Shane and Contractor62)

Ovung A, Bhattacharyya J

J., Ceradini, D