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Thereafter, DMSO and Sorenson's glycine buffer (0.1 M glycine and 0.1 M NaCl, adjusted to pH 10.5 with 0.1 mM NaOH) were added to solubilise the dye before absorbance was read at 570 nm using a BioTek ELx800 plate reader and Gen 5 software for data acquisition

Ten grams of the fresh fungal biomass were minced in liquid N 2 , dispensed in 50 ml of 50 mM potassium phosphate buffer of pH 6.5 with 1 mM PMSF, and 1 mM EDTA, the homogenate was vigorously shacked for 5 min, centrifuged at 6000 rpm for 10 min, the supernatant was used as source of the crude enzyme [19, 23, 36]

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