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Tissues were dissected, post-fixed for 3 h in 4% PFA at room temperature 1820 2 C cryoprotected in 30% (w/v) sucrose in Tris-Azide buffer (40 mM Tris, 10 mM phosphate, 0.7% (w/v) NaCl, 0.05% (w/v) NaN 3 ) at 4 C, then embedded in optimal cutting temperature (OCT) compound, rapidly frozen in isopentane on dry ice, and stored at 80 C until use

xCT-overexpressing MEF were exposed to 100 M of each inhibitor for 5 min

It is integral to DNA (deoxyribonucleic acid) repair and protein synthesis, ensuring genetic integrity and proper cellular function

There is growing evidence that immune dysregulation and neuroinflammation are central to its pathogenesis, with the microbiota-gut-brain (MGB) axis playing a critical role