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The gel was transferred onto a polyvinylidene fluoride (PVDF) membrane using the iBlot 2 Dry Blotting System using preprogramed voltage combinations (i.e., 20 V for 1 min, 23 V for 4 min, and 25 V for 2 min)

However, it is important to acknowledge that this superiority was not consistent across all biochemical parameters: markers such as SOD, CAT, MDA, IL-1, SLC7A11, and Nrf2 did not differ significantly between the combination and TMZ alone

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Irradiation with a single dose of 5 Gy led to a pronounced and sustained reduction of GSH that was still visible 12 h after irradiation, whereas GSH levels of irradiated TrC1 AKT-E17K cells at 12 h after irradiation were similar to untreated control cells ( Figure 1B )

According to the incidence, prevalence, and social impact up to 349.2 million individuals were affected by major neurological disorders worldwide (Ding et al., 2022