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Lingyu, M., Hongguang, L., Mingdong, Z., Na, L

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Our research integrates classical microbiology, whole genome sequencing, metagenomics, emerging molecular detection approaches, and targeted phenotypic analyses

Immunofluorescence, confocal microscopy image acquisition and colocalization analysis For the immunofluorescence analysis of endogenous proteins, RAW 264.7 murine macrophages (ATCC, TIB-71) were seeded on untreated coverglass using a four-chamber 35-mm dish with 20-mm microwells (Cellvis, D35C4-20-1.5-N), incubated at 37 C for 30 min, then fixed with 3.7% paraformaldehyde for 8 min, permeabilized with 0.01% saponin and blocked with 1% BSA in PBS