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Deparaffinization was performed by incubating the slides twice in 100% xylene for 15 min, followed by rehydration through a graded ethanol series (100%, 95%, and 70%) and finally distilled water, with each step lasting 5 min

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Consistent with this, intrathecal infusion of FGF-2 or EGF, which increase number of astrocytes and ependymal cells, have been shown to improve functional recovery in spinal cord injury [64], whereas astrocyte depletion significantly increase leukocyte infiltration, blood brain barrier disruption, and damage [49,51]

Castor oil works best in cold or dry climates because its heavier texture helps lock in moisture and prevent the skin from drying out in the winter months