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(I) HEK293 cells were transfected with the expression plasmid of Flag-tagged TLR4, lysed and treated with GST-HBeAg, and then incubated with glutathione beads for the pull-down experiment

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The results revealed a significant increase in the expression levels of KIM-1, a biomarker of renal tubular injury during AKI, whereas the expression levels of GSTT1 and GSTM1 were markedly decreased ( Figure 2 )