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The PCR conditions were: initial 4 min denaturation at 95C, then 32 cycles of 95C for 40 s, 58C for 30 s and 72C for 60 s

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Nature 468 (7327), 11151118

6 Patients at Increased Risk Because the mechanism of acetaminophen toxicity occurs via the formation of NAPQI, any factors that influence the availability of metabolic enzymes will therefore affect toxicity