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The cells were pretreated with caffeine (256 or 512 M) for 1 h and subsequently irradiated with UVB lamp for 5 min, then lysed by adding KDalert lysis buffer from the kit, and finally immunoprecipitated with SIRT3 antibody

Vesicles 4 , 27066 (2015)

Subjects who withdrew from the randomized withdrawal period and who did not provide efficacy data at their last on-treatment visit were classified as treatment failures (Study 6, Figure 5)