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For extraction, common organic solvents like mixtures of hexane with acetone, ethanol, and methanol or ethyl acetate with acetone, ethanol, and methanol have been used [8, 9]

Arunachalam, K., & Alzahrani, T

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Purified CFE Systems While the previous subsections have covered extract-based CFE systems, in vitro transcription and/or translation can also be recapitulated using only purified components (Figure 5), allowing the reaction composition to be precisely defined

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