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2.3.2 Toll-like receptor agonists TLR agonists (including TLR7 agonists, TLR-7/8 agonists, and TLR9 agonists) can also polarize M2-like TAMs to M1-like TAMs in addition to iron-based nanoparticles, but their application in vivo is limited by the fact that TLRs are easily cleared by nucleases in circulation and can also trigger inflammatory responses through non-specific stimulation ( 3 O 4 NPs and the TLR7 agonist imiquimod (R837) coated with lipopolysaccharide (LPS)-treated macrophage membranes can target TAMs, in which PIR@M can polarize TAMs from M2 to an anti-tumor M1 phenotype by the synergistic effect of Fe 3 O 4 NPs and R837 thereby attenuating immunosuppressive TME and activating the anti-tumor immune response (39)