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Afterwards, each slide was treated with 100 L of 0.25 mg ml 1 CMA3 (Sigma Co.) in a dark and humid condition for 20 min

The expansion of a G4C2 (GGGGCC) hexanucleotide repeat in the non-coding region leads to the production and accumulation of dipeptide repeat proteins in neurons (Maor-Nof et al., 2021)

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We identified strong localization of mNG::HttQ74 to IFD-1 and IFD-2 inclusions in the touch neuron soma when mNG::HttQ74 is expressed at levels at which visible aggregation is present (Fig