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Chromatographic separation was achieved using an Acquity C18 column (3 m, 50 2.1 mm, Thermo Scientific, Waltham, MA) with a guard column, heated to 40 C, and a binary gradient at a flow rate of 0.5 mL/min

Taken together, current literature supports further investigation but underscores the need for well-controlled human clinical trials to determine safety, dosing, and efficacy

Pflgers Archiv European Journal of Physiology

However, this large number warrants important context: 35 of the 36 studies included in the systematic review were preclinical (conducted in laboratory or animal models), and only one clinical study met the review's inclusion criteria