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Upon exposure to cisplatin-induced cellular DNA damage, p53 is phosphorylated, and the proapoptotic protein BAX undergoes structural modifications and alters mitochondrial membrane integrity, causing the activation of p53 upregulated modulator of apoptosis- and Ca 2+ -independent phospholipase A2

Subsequently, the blots proceeded through a second round of immunodetection by agitating for 45 min at 50C in stripping buffer (2% SDS, 0.8% -mercaptoethanol (ME), and 62.5 mM Tris-HCl, pH 6.8) 32,56,62 followed by re-probing with HRP-labeled anti-HGF N -terminal domain mAb (1:2500 dilution in CanGetSignal solution 1) to monitor HGF -chain amount on the blots as loading controls

Once reconstituted, researchers can explore its effects in cell cultures, biochemical assays, and other laboratory models that investigate cellular responses and signaling behavior

Angal S, King DJ, Bodmer MW, Turner A, Lawson AD, Roberts G, et al

Senolytic Peptide Researched for clearing senescent cells