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Step iii To a stirred solution of methyltriphenylphosphonium bromide (4.60 g, 12.89 mmol) in THF (30 mL) at 78 C

This can lead to chafing, which causes hyperpigmentation

The first step, catalyzed by glutamate-cysteine ligase (GCL), is rate-limiting and regulated by GSH (competitive inhibitor) and by cysteine availability (Lu, 2013)