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Osteoclasts are huge multinucleated cells derived from monocyte/macrophage cell lines, filling between inflammatory synovial tissue and the surface of bone joints

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Cell culture, treatment, and transfection Human HEK293T and HT1080 cells were obtained from the American Type Culture Collection (ATCC) and maintained (37 C, 5% CO 2 ) in DMEM high-glucose medium (Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), penicillinstreptomycin (100 U/mL, Gibco, 15140148), sodium pyruvate (1 mM, Gibco, 11360070), and MEM nonessential amino acids (1, Gibco, 11140050)