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Real-time quantitative PCR Following drug treatments, total RNA was extracted from THP-1 cells using Trizol reagent (Biosharp, BS259A) and subsequently reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1691)

meditteranei , a perchlorate-reducing microorganism (Oren et al., 2014), also demonstrated the enzymes ability to reduce perchlorate (Martnez-Espinosa et al., 2015)

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