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Statements Author contributions LJW: Conceptualization, Visualization, Writing review & editing

Mutation of Tyr 180 to Ala reduced activity by ~80%, even though the side chain is far from the active site, so it is unlikely that Tyr 180 is involved in catalysis

Additionally, the research determined the semi-lethal concentration (LD50) and established the safe dosage range of CGA in Kunming (KM) mice through in vivo acute toxicity testing via tail vein injection