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Recycled citrulline synergizes with aspartate to synthesize arginine for NOS reuse in turn by upregulating argininosuccinate synthase and argininosuccinate lyase in M1 macrophages through the citrullineNO cycle [58]

Almost concurrently, the glutaminase II pathway was identified in prostate cancer cells, and expression of the GTK and -amidase genes in this pathway has been shown to be more upregulated with increased cancer cell invasiveness (125)

Therefore, it is crucial to apply antibiotics cautiously, and properly (in the time of application as well as dose) using the proper guidelines to reduce the negative effects on the gut microbiota of fish and utilization of alternative strategies will be important rather than depend entirely on antibiotics for treatment of aquatic animal diseases

doi:10.1111/jebm.12588 Zhang N, Zhou Z, Chi X, et al