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The lenses were subsequently cultured for 23 days in a medium containing either galactose alone or a galactose medium with various concentrations of glutamate (1 mM, 5 mM, 20 mM, and 40 mM) to determine the glutamate concentration at which the opacity was reduced (Fig

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Materials and methods Clinical samples of hepatocellular carcinoma patients Human hepatocellular carcinoma (HCC) cancer tumor tissues and matched adjacent tissues ( n = 20) were collected from Cancer Hospital Affiliated to Harbin Medical University for qRT- PCR, western blot and Eliza assays

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Healthy volunteers are mainly involved in phase 1 of the research process