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The Wnt/calcium pathway , regulating calcium release to control cell adhesion and targeted gene expression

doi: 10.1002/cti2.70011

During disease remission, the restoration of gut microenvironment homeostasis leads to demand-dependent reductions in the abundance of Clostridium leptum and Clostridium cluster XI , classified as potential conditional beneficial bacteria, which align with the principle of dynamic gut microbial balance and may not be directly linked to therapeutic efficacy

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Briefly, the lentiviral vector containing inserts of interest and three packaging system plasmid (Rev,Gag and VSVG) were mixed and transfected into 293 T cells with EndoFectin-Lenti reagent (GeneCopoeia, USA), and then the virus containing culture medium was collected 48 h after transfection and spun for 10 min at 3000 r.p.m