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Afterward, a mixture of methanol-saturated petroleum ether and petroleum ether-saturated methanol (1:1, by volume) was sequentially added to the total fat, followed by sequential addition of petroleum ether-saturated methanol and methanol-saturated petroleum ether mixture (6:1, by volume) after shaking and homogenization

Hearts were digested for 20 min at 37 C

CrossRef Beneficial Effects of Pomegranate Peel Extract and Probiotics on Pre-adipocyte Differentiation Valeria Sorrenti, Cinzia Lucia Randazzo, Cinzia Caggia, Gabriele Ballistreri, Flora Valeria Romeo, Simona Fabroni, Nicolina Timpanaro, Marco Raffaele, Luca Vanella Frontiers in Microbiology.2019;[Epub] CrossRef Visfatin