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5 Microbiota transplantation Microbiota transplantation (MT), particularly fecal microbiota transplantation (FMT), involves transferring gut microbiota from healthy donors into individuals with ASD to correct dysbiosis and modulate the MGBA

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Protoplasts were collected via centrifugation at 100 g for 2 min and lysed by vortexing using 300 l of IP buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% Triton X-100 and protease inhibitor cocktail) followed by centrifugation at 12,000 g for 10 min at 4 C

These compounds exhibit a broad spectrum of activity, including the elimination of various pathogens, as well as the potential to activate anti-inflammatory cells and recruit them from the bloodstream to damaged tissues [17]