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Description

Pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method

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Coupled activity assay with Cp1B and Cp1D The coupled activity assay for Cp1B with Cp1D was typically performed in 100 l of 50 mM sodium phosphate buffer (pH 8.0) containing 25 M Cp1B, 25 M Cp1D, 5 mM ()- t -ES, 2.5 mM amino acid, 5 mM amine, 10 mM ATP cofactor and 10 mM MgCl 2

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Thus, APC/C CDH1 is likely the physiological E3 ubiquitin ligase for PIN1, and its activity is primarily inhibited by phosphorylation of CDH1 in cancer cells