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Because the liver stores several years' worth of B12, symptoms often creep in gradually, which is one reason the condition is underdiagnosed

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The collected diffraction patterns were subjected to background correction and standard smoothing, and the resulting data were analyzed using DIFFRAC.EVA V4.1 software, comparing them to both the PDF-2 commercial database and an in-house reference library

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Acknowledgments The authors are grateful to the Core Facility Center, Office of the Research and Development, Taipei Medical University, for its great support in TOF-SIMS analysis