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Cells were trypsinized, collected and lysed in 50 mM Tris-HCl, pH 8, containing 1% SDS and protease inhibitor (1x complete mini protease inhibitor cocktail) for 20 min on ice, followed by probe sonication (4 15 s)

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Use of the pharmamers of the present invention for immune monitorering assays such as MHC multimer assays, ELISPOT, CFC and other assays involving formation of MHC-peptide complexes are also part of the present invention

The estimates suggest that approximately one in five men or women develop cancer in a lifetime, whereas around one in nine men and one in 12 women die from it ( In recent years, Traditional Chinese Medicine (TCM) has gained recognition for its role in cancer treatment, with several anti-tumor compounds identified in traditional remedies ( Compared with other anti-tumor compounds derived from Chinese herbal medicine, glabridin shows significant advantages

95 HaraT.NakamuraK.MatsuiM.YamamotoA.NakaharaY.Suzuki-MigishimaR.et al