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Description

Mf were cleaned and filtered in RPMI 1640 culture media (Gibco, Thermo Fisher Scientific) using PD-10 desalting columns (GE Healthcare) supplemented with 2 mM L-glutamine, 25 mM Hepes, 100 U/mL penicillinstreptomycin (Thermo Fisher Scientific) and 2.5 mg/mL amphotericin B (Thermo Fisher Scientific) at 37 C

Cold vial + warm air = condensation of moisture inside

Likewise, homocysteine is also HIGH if you are B12 deficient

Therefore, distinguishing between the DNA damage response, redox signaling activation, or even their combination in triggering p53 activation is difficult (Shi And Dansen 2020a, 2020b)