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Cys are the elements most sensitive to fluctuations of the cellular redox environment, undergoing different types of chemical modifications in response to such variations

Doxycycline, a tetracycline antibiotic, interferes with bacterial protein synthesis by interacting with the 30S subunit of the bacterial ribosome, inhibiting bacterial growth, and is thought to possess anti-inflammatory effects

To investigate whether the differences in the concentration of total soluble protein and the yields were statistically significant for the different combinatorial strategies of purification, One-way ANOVA was performed with Tuckeys method that uses studentized range distribution [36]

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