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Bone Marrow-derived macrophages (BMDMs) culture and in vitro assays BMDMs were isolated from female C57BL/6 J mice and cultured in DMEM (Cell Clone) supplemented with 10% FBS (Cell Clone), 30 ng/ml macrophage colony-stimulating factor (M-CSF, BioLegend), 2 mM L-glutamine (Sigma Aldrich), 1 mM sodium pyruvate (Sigma Aldrich), 10 mM HEPES buffer (Sigma Aldrich), and 1% penicillin/streptomycin (Sigma Aldrich) at 37 C for 6 d