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Reduced roGFP2 was obtained after a 20 min treatment with 10 mM DTT, which was subsequently removed on a ZebaTM Spin Desalting Column (Thermo Scientific)

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This process allows us to propose the three sites for substrate interaction: site I in H2 (Lys 67), site II in H4 (Arg 151, Gln 152), and site III in H6 (Arg 262, Supplementary Figure S1 )

De la maltodextrine ou du dextrose de 60 g est amplement suffisant

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