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The cells were lysed, and the supernatant was collected by centrifuging at 10,000 x g at 4C for 10 min and then determined for reduced glutathione (GSH) and oxidized glutathione (GSSG) levels using GSH and GSSG assay kit (Beyotime Institute of Biotechnology) according to the manufacturers instruction

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Malabsorption Malabsorption of B12 is prevalent in people with gastrointestinal (GI) disorders and GI surgeries

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Furthermore, zincs antioxidant properties contribute to its protective role against oxidative stress, a major driver of cellular aging and tissue damage in older adults [17]