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1 ug of RNA was reverse transcribed using the Superscript III Reverse Transcriptase kit (Invitrogen) according to the manufacturers instructions

Superoxide is rapidly removed by high concentrations of scavenging enzymes called superoxide dismutases (SOD) with distinct isoenzymes located in the mitochondria, cytoplasm, and extracellular compartments

186 PintoJ.SkjefteM.Alonso-PadillaJ.Lozano BeltranD

He made important discoveries in many different fields of chemistry physical, structural, analytical, inorganic, and organic chemistry, as well as biochemistry

Initially (untreated cells for initial time point) or after 4 days (with varying treatment conditions), 40 L of Cell Titer Glo reagent (Promega) was added to each well, mixed briefly, and the luminescence read on a luminometer (Molecular Devices)