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Bioinformatics analysis Raw transcriptomic sequencing data were processed using the nf-core RNA-seq v3.12.0 pipeline from the nf-core collection of community-curated, reproducible workflows 59

In vitro testing on A549 and B16 cell lines identified 8-(pyrrolidin-1-ylmethyl)4-trifluoromethyl ESC as the optimal derivative, demonstrating 20-fold enhanced antiproliferative activity and threefold prolonged half-life in human liver S9, with structural analysis revealing that nitrogen-containing groups at C-8 substantially enhance both metabolic stability and anticancer potency (Pan et al

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This ensures that the vitamins adhere to specific standards and are generally safe to take

Mohan RR, Rodier JT, Sharma A