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Intranasal delivery of peptides to the brain relies primarily on extracellular passive transport from the olfactory region of the nasal cavity [17, 18, 19]

Sirius red staining Sections were stained with saturated picric acid solution containing 0.1% Sirius Red for 15 min at room temperature, ensuring complete coverage of the muscle tissue

In severe PEM, there is a marked decline in the hepatic synthesis of transport proteins including thyroxine-binding globulin (TBG), thyroxine-binding pre-albumin (TBPA), and albumin [32]