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After 1 h infection, cells were washed three times with fresh culture medium and incubated for 24 h in DMEM supplemented with 5% (v/v) fetal calf serum, 10 g m1 1 gentamycin (to kill extra-cellular bacteria) and 1.5 mg ml 1 cefotaxime (to kill growing intra-cellular bacteria)

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Reduced lysosomal Ca 2+ levels, probably reflecting excessive calcium efflux, are also seen in forms of PD caused by mutations in GBA1 (refs

Equity of access is a problem because these medications are costly and there have been previous global shortages because of overdemand