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After homogenization, the brain tissues were centrifuged at 12,000 rpm at 4C for a quarter

Systemic injection of human ExerVs during hindlimb unloading enhances recovery of Type I fibers during reloading Based on the results above, we speculated that ExerV treatment during disuse may alter the ECM environment in a beneficial manner that may promote fiber growth during reload 33

Eluted protein was further purified with SEC (Superdex 75, Cytiva) in the SEC buffer (20 mM Hepes pH 7.5 and 400 mM NaCl)