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(B6, especially in the pyridoxine form, is one of the few B vitamins that can lead to toxicity when used in excess.) Threonine is also important to gut barrier integrity in the small intestine, and I have read some reports of it helping with minimizing reactions to gluten, although I have not studied this in great depth yet

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IF analysis showed strong cytoplasmic retention of NUPR1 protein and a clear reduction of nuclear NUPR1 in treated cells, confirming target engagement in our system (Fig

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