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DNase I digestion was also applied

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Furthermore, because of the complex heterogeneity of GBM, and unique characteristics of the bloodbrain barrier (BBB) and bloodbrain tumor barrier (BBTB) structures in the brain, most chemotherapeutic agents cannot smoothly reach the lesion and accumulate to a sufficient concentration in the tumor microenvironment (TME), severely limiting GBM treatment [7]

Never let it bake in direct sunlight or sit on a piece of equipment that generates heat