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Advances in bone preservation: identifying possible collagen preservation using sulfur speciation mapping

The dissolved oxygen concentrations in bottom water were higher in May and June than in July

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It demands understanding the difference between variables that affect pharmacology (storage temperature, reconstitution sterility, dosing intervals) and variables that merely affect convenience (morning versus evening injection)

After culture for 6 h at 20C, cells were harvested by centrifugation (15 min, 6000 g, 4C), re-suspended in PBS (15 ml per pellet from 1 L of culture), and subsequently lysed by sonication (cycle of 30 0.5 s pulses at a power output of 70% per pellet from 1 L of culture) using a Branson Sonifier Digital 450 (Branson Ultrasonics, Danbury, CT, USA)