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The relA was attenuated for survival in calf and goat tissue and it also decreased the capacity of the infection strain to colonize in tissue, evident by reduced number of positive tissue sites in vaccinated animals ( Screening a library of MAP transposon mutants in calf infection model categorized MAP coding regions into 683 essential, 304 growth-defect, 3277 non-essential and 92 growth-advantage coding regions ( IcL , MAP1643) and one growth-defect gene ( BacA , MAP1531c) ( Figure 1A )

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Indeed, since proteolytic enzymes prefer the natural L-configuration rather than the D-one, it is well-documented that replacing L-amino acids with their D-enantiomers, incorporating methylated amino acids, or introducing -amino acids and peptoid residues are among the usual approaches to prolonging the plasma half-life