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Revenkova, E., Herrmann, K., Adelfalk, C

Immunofluorescence microscopy Sections of cortex were stained for the astrocyte marker, glial fibrillary acidic protein (GFAP), and nuclei were stained with DAPI, using a standard immunohistochemistry protocol

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VNO with exposed vomeronasal neuroepithelium was put in a recording chamber filled with Ringers solution (140 mM NaCl, 5.6 mM KCl, 5 mM HEPES, 2 mM pyruvic acid sodium salt, 1.25 mM KH 2 PO 4 , 2 mM CaCl 2 , 2 mM MgCl 2 , 9.4 mM D-glucose (pH 7.4))