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After the indicated incubation times, reactions were quenched by adding 1LmL cold KPBS, followed by two additional washes in 1LmL cold KPBS using the multiLchannel pipette

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Stanulla M, Schrappe M, Brechlin AM, Zimmermann M, Welte K

Comparisons between the transgenic GSAAT1 substitution mutants and wild-type GSAAT1 expressed in the gsa2 background revealed a slightly reduced ALA synthesis capacity of leaf discs and in planta GSAAT activity of the leaf extracts only for the GSAAT(C190S) line ( Supplementary Figures 3C, E )

Accordingly, inhibition of SNAI1 in mice after induction of unilateral ureteral obstruction resulted in a substantial reduction of renal fibrosis and inflammation (80)