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Editing efficiency was determined on bulk-edited cell populations by targeted PCR of the Cas9-targeted region and secondary PCR using overhang sequences (Table S1), followed by Illumina MiSeq, as previously described (Aubrey et al, 2015)

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3B and 3C are as follows: the sequence for sh263-VDAC3 is shown in SEQ ID NO:1, the sequence for sh548-RPL8 is shown in SEQ ID NO:2, the sequence for sh440-ATP5G3 is shown in SEQ ID NO:3, the sequence for sh978-TTC35 is shown in SEQ ID NO:4, the sequence for sh2326-IREB2 is shown in SEQ ID NO:5, the sequence for sh1776-ACSF2 is shown in SEQ ID NO:6, and the sequence for sh332-CS is shown in SEQ ID NO:7

Reactive oxygen species have been associated with many disease categories, including cancer

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