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However, optimal effects such as improved sleep, mood, or recovery often require multiple sessions over days or weeks, especially for chronic fatigue, addiction support, or anti-aging protocols

Rodent studies from the Kozlovskaya and Semenova groups at the Institute of Molecular Genetics report increased 5-hydroxyindoleacetic acid (5-HIAA) to serotonin ratios in the hypothalamus and striatum, suggesting accelerated serotonin turnover rather than raw 5-HT elevation

PLoS ONE 10 , e0122124 (2015)

doi: 10.1038/hr.2009.193